Mitotic cell death in BEL-7402 cells induced by enediyne antibiotic lidamycin is associated with centrosome overduplication

Figure 4 Immunofluorescence analysis of centrosomes and mitotic spindles in BEL-7402 cells at 72 h after treatments with 0. 5 nmol/L lidamycin for 2 h. Cells grown on coverslips were fixed in paraform and doubly labeled with antibodies to α-tubulin (red) and centrin (green). Localization of centrosome on superimposed image showed yellow. A: Untreated BEL-7402 cells; B, C: 0.5 nmol/L lidamycin treated BEL-7402 cells. Arrows in B, C indicated the cells with multiple centrosomes, multipolar mitotic spindle or chromosome missegregation.