Basic Research
Copyright ©The Author(s) 2004.
World J Gastroenterol. Sep 15, 2004; 10(18): 2706-2710
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2706
Table 1 Primer sequences for PCR and amplification conditions for each target gene
Primer (base)SequenceAmplification conditions
Fas 4145’-GAATGCAAGGGACTGATAGC-3’Denaturation at 94 °C for 45 s,
5’-TGGTTCGTGTGCAAGGCTC-3’Annealing at 55 °C for 30 s and synthesizing
at 72 °C for 1 min for 25 cycles
FasL 2395’-GGAATGGGAAGACACATATGGAACTGC-3’Denaturation at 94 °C for 45 s,
5’-CATATCTGGCCAGTAGTGCAGTAATTC-3’Annealing at 55 °C for 30 s and synthesizing
at 72 °C for 1 min for 33 cycles
Bcl-25255’-TATGATAACCGGGAGATCGTGATC-3’Denaturation at 94 °C for 45 s,
5’-GTGCAGATGCCGGTTCAGGTACTC-3’Annealing at 60 °C for 30 s and synthesizing
at 72 °C for 1 min for 33 cycles
Bax 3105’-GACACCTGAGCTGACCTTGG-3’Denaturation at 94 °C for 45 s,
5’-GAGGAAGTCCAGTGTCCAGC-3’Annealing at 60 °C for 30 s and synthesizing
at 72 °C for 1 min for 30 cycles
β-actin 6605’-CCAACCGTGAAAAGATGACC-3’Changed according to different target genes
5’-CAGGAGGAGCAATGATCTTG-3’