Copyright
©The Author(s) 2004.
World J Gastroenterol. Sep 15, 2004; 10(18): 2706-2710
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2706
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2706
Primer (base) | Sequence | Amplification conditions |
Fas 414 | 5’-GAATGCAAGGGACTGATAGC-3’ | Denaturation at 94 °C for 45 s, |
5’-TGGTTCGTGTGCAAGGCTC-3’ | Annealing at 55 °C for 30 s and synthesizing | |
at 72 °C for 1 min for 25 cycles | ||
FasL 239 | 5’-GGAATGGGAAGACACATATGGAACTGC-3’ | Denaturation at 94 °C for 45 s, |
5’-CATATCTGGCCAGTAGTGCAGTAATTC-3’ | Annealing at 55 °C for 30 s and synthesizing | |
at 72 °C for 1 min for 33 cycles | ||
Bcl-2525 | 5’-TATGATAACCGGGAGATCGTGATC-3’ | Denaturation at 94 °C for 45 s, |
5’-GTGCAGATGCCGGTTCAGGTACTC-3’ | Annealing at 60 °C for 30 s and synthesizing | |
at 72 °C for 1 min for 33 cycles | ||
Bax 310 | 5’-GACACCTGAGCTGACCTTGG-3’ | Denaturation at 94 °C for 45 s, |
5’-GAGGAAGTCCAGTGTCCAGC-3’ | Annealing at 60 °C for 30 s and synthesizing | |
at 72 °C for 1 min for 30 cycles | ||
β-actin 660 | 5’-CCAACCGTGAAAAGATGACC-3’ | Changed according to different target genes |
5’-CAGGAGGAGCAATGATCTTG-3’ |
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Citation: Wang XZ, Zhang SJ, Chen YX, Chen ZX, Huang YH, Zhang LJ. Effects of platelet-derived growth factor and interleukin-10 on Fas/Fas-ligand and Bcl-2/Bax mRNA expression in rat hepatic stellate cells
in vitro . World J Gastroenterol 2004; 10(18): 2706-2710 - URL: https://www.wjgnet.com/1007-9327/full/v10/i18/2706.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i18.2706