Differentiation of dermis-derived multipotent cells into insulin-producing pancreatic cells in vitro

Table 1 DMC gene lists (partial) associated with diverse cellular lineages

Cell lineage	Representative examples of associated genes
A: Pancreatic cell	Pancreatic eukaryotic initiation factor 2 alpha-subunit kinase (PEK), pancreatic secretory trypsin inhibitor-like protein (PSTI), pancreatic islet cDNA Rattus norvegicus cDNA similar to rapamycin-binding protein FKBp-13, insulin II gene, IRS-1 mRNA for insulin-receptor,mRNA for glucose-dependent insulinotropic polypeptide
B: Neural cell	Neural visinin-like Ca2 + -binding protein type 3, neuron-specific enolase, neuronal nitric oxide synthase, GABA receptor-associated proteins, NCAM, brain-derived neurotrophic factor (BDNF), syntaxin 7, glial fibrillary acidic protein (GFAP), adrenomedullin, myelin protein SR13 (growth-arrest-specific Gas-3 homolog), neurotrimin; secretogranin II, synapse-associated protein 102, latexin, kexin-like protease PC7A, N-methyl-D-aspartate (NMDA) receptor
C: Epithelial cell	Epithelial membrane protein 1, epithelial cell transmembrane protein antigen precursor, epidermal growth factor precursor, heparin-binding EGF-like growth factor , mucin, parathymosin and thy mosin 4 and 10
D: Hepatocyte	Liver glycogen phosphorylase enzyme, liver IL-6 receptor ligand binding chain, liver specifc tran scription factor LF-B, liver nuclear protein P47, liver aL-Fucosidase, hepatocyte nuclear factor 3a (HNF-3beta)

Expression of a single gene can’t define the phenotype of a particular cell type. Simultaneous expression of a panel of lineage-related genes in single isolated cell was viewed as the harbinger of a potential cell type. Representative examples of genes corresponding to each cell lineage are outlined in the above table.