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©The Author(s) 2004.
World J Gastroenterol. Sep 1, 2004; 10(17): 2493-2497
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2493
Published online Sep 1, 2004. doi: 10.3748/wjg.v10.i17.2493
Table 1 Oligonucleotide primers used for cagA, vacA and iceA genotyping
Region detected | Primer designation | Primer sequence | Size of PCR product (bp) | References |
s1 and s2 | VA1-F | 5’ATGGAAATACAACAAACACACC3’ | 259/286 | 14 |
VA1-R | 5’CTGCTTGAATGCGCCAAACTTTATC3’ | |||
s1a | SS1-F | 5’GTCAGCATCACACCGCAAC3’ | 190 | 20 |
s1b | SS3-F | 5’AGCGCCATACCGCAAGAG3’ | 187 | 20 |
s1c | S1C-F | 5’CTYGCTTTAGTRGGGYTA3’ | 213 | 26 |
M1 | VA3-F | 5’GGTCAAAATGCGGTCATGG3’ | 290 | 20 |
VA3-R | 5’CCATTGGTACCTGTAGAAAC3’ | |||
M1T | m1T-F | 5’GGTCAAAATGCGGTCATGG3’ | 290 | 14 |
m1T-R | 5’CTCTTAGTGCCTAAAGAAACA3’ | |||
M2 | VA4-F | 5’GGAGCCCCAGGAAACATTG3’ | 352 | 20 |
VA4-R | 5’CATAACTAGCGCCTTGCAC3’ | |||
iceA1 | iceA1F | 5’GTGTTTTTAACCAAAGTATC3’ | 247 | 21 |
iceA1R | 5’CTATAGCCASTYTCTTTGCA3’ | |||
iceA2 | iceA2F | 5’GTTGGGTATATCACAATTTAT3’ | 229 | 21 |
iceA2R | 5’TTRCCCTATTTTCTAGTAGGT3’ | |||
lcagA | lcagAD008 | 5’ATAATGCTAAATTAGACAACTTGAGCGA3’ | 297 | 8 |
lcagAR008 | 5’TTAGAATAATCAACAAACATCACGCCAT3’ |
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Citation: Lin HJ, Perng CL, Lo WC, Wu CW, Tseng GY, Li AFY, Sun IC, Ou YH.
Helicobacter pylori cagA ,iceA andvacA genotypes in patients with gastric cancer in Taiwan. World J Gastroenterol 2004; 10(17): 2493-2497 - URL: https://www.wjgnet.com/1007-9327/full/v10/i17/2493.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i17.2493