4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells

Figure 8 PSCs were treated with HNE (at 1 µmol/L , lane 2) in the absence or presence of SB203580 (at 25 mmol/L , lane 3), SP600125 (at 10 µmol/L, lane 4), or U0126 (at 5 µmol/L, lane 5). After 48-h incubation, type I collagen released into the cultute supernatant was quantified by enzyme-linked immunosorbent assay. The collagen levels in each sample were normalized to the cellular DNA content. The results are expressed as a per-centage of the untreated control (“Con”). Data are shown as mean ± SD (n = 6). ^bP < 0.01 vs control (serum-free medium only, lane 1). ^dP < 0.01 vs HNE only (lane 2).