4-hydroxy-2, 3-nonenal activates activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells

Figure 6 PSCs were treated with HNE (at 0, 1, or 5 μmol/L ) in the absence or presence of PDGF (at 25 ng/mL) in serum-free medium. After 24-h incubation, cells were labeled with BrdU for 3 h. Cells were fixed, and incubated with peroxidase-conju-gated anti-BrdU antibody. Then the peroxidase substrate 3,3’, 5,5’-tetramethylbenzidine was added, and BrdU incorporation was quantitated by differences in absorbance at wavelength 370 min 492 nm (“A.”, optical density). Data are shown as mean ± SD (n = 6). ^bP < 0.01 versus control (serum-free medium only), N.S.: not significant.