Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential

Figure 2 Results of secondary PCR amplification. Lanes 1-3: Product of primary PCR amplification, Lane 4: Secondary PCR amplification product of PCR control cDNA, Lane 5: Second-ary PCR amplification product of unsubtracted cDNA ,Lane 6: Secondary PCR amplification product of subtracted cDNA.