Hepatitis B virus X gene induces human telomerase reverse transcriptase mRNA expression in cultured normal human cholangiocytes

Figure 4 HBx protein expression in transferred HBECs assayed by ultrasensitive immunocytochemistry. The blank vector and OPTI-MEN transfected HBECs showed no expression of HBx protein (A, C), but pale brown positive signals scattered in pCMV- X vector transferred HBECs (B). Immunocytochemis-try (S-P methods, × 200).