Hepatitis B virus X gene induces human telomerase reverse transcriptase mRNA expression in cultured normal human cholangiocytes

Figure 1 Transfection efficiency evaluated by EGFP-express-ing cells in the HBx gene transfected cell cultures and empty vector control. pEGFP 0.1 μg and pCMV-X (or pcDNA3) 2.9 μg were co-transfected into HBECs by Lipofectamine. Thirty-eight hours after transfection. EGFP-expressing cells were visible under fluorescence microscope in the HBx gene transfected cell cultures (A) and empty vector control (B), but not in the blank control (Under fluorescence microscope × 200).