Expression of cyclooxygenase-2 in human esophageal squamous cell carcinomas

Figure 1 Semiquantitative result of RT-PCR analysis of COX-2 mRNA expression in squamous carcinoma tissues and matched normal tissues of the esophagus. A: Data are expressed as the fluorescence values of COX-2 band in tumor and nontumorous tissue samples from 30 patients with esophageal squamous cell carcinoma. B: Representative results of semiquantitative RT-PCR, indicated COX-2 mRNA was found in esophageal squamous cell carcinoma samples (T), while in surrounding normal tissue (N), COX-2 mRNA could not be detected. The coamplified GAPDH gene served as an internal control. PCR product sizes are 314 bp for COX-2 and 340 bp for GAPDH. M indicates DNA marker. The samples in lanes 1N and 1T, 2N and 2T, 3N and 3T, 4N and 4T are paired samples from 4 patients, respectively.