In vitro anti-coxsackievirus B3 effect of ethyl acetate extract of Tian-hua-fen

Table 2 Protective effect of ethyl acetate extract on HeLa cells shown in the value of A₆₀₀

Dilution	1:256	1:512	1:1024	1:2048	1:4096	1:8192
Value of A600	1.09 ± 0.017	1.02 ± 0.122	0.89 ± 0.060	0.91 ± 0.039	0.83 ± 0.048	0.77 ± 0.028

The A₆₀₀ value of the cells co-cultured only with RPMI1640 was 1.32±0.02; the A₆₀₀ value of the cells co-cultured only with 10^-5 CVB_3m was 0.22 ± 0.026; and the A₆₀₀ value of the cells co-cultured only with extract at concentration of 1:256 mg/mL was 1.21 ± 0.042. As shown in the table the value of A₆₀₀ of the cells treated with extract is higher than that of the cells infected with CVB_3m while not treated with the extract (^bP < 0.01), indicating the protective effect of the extract on’ HeLa cells from CVB_3m infection.