Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration

Figure 6 SDS-PAGE analysis (150 g/L) for expression (panel A) and immunoblot of fusion pollen allergen D106 (panel B). Panel A: 1. Lysate pellet of induced cells; 2. Cells without IPTG induction; 3. Supernatant of induced cell lysate; 4. Purified fusion pollen allergen D106; M. Broad range prestained SDS-PAGE standards (Bio-Rad, Hercules, CA, USA). Panel B: Fusion protein D106 was blotted with 8 serum samples from pollen allergic patients. Each strip represents one serum sample.