Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration

doi:10.3748/wjg.v10.i14.2103

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Jul 15, 2004 (publication date) through Jan 4, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 15, 2004; 10(14): 2103-2108
Published online Jul 15, 2004. doi: 10.3748/wjg.v10.i14.2103

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Figure 3 Phylogenetic relationships between newly obtained pollen allergen D106 (Amb a 8(D106)) and its cognates from GenBank as inferred from CLUSTAL W (1. 82) alignment of the amino acid sequences. Amb a 8(D106) shared a homology as highly as 54%-89% with different pollen allergens (from mugwort CAD12862 to apple Q9XF41) and as 79%-89% with different food allergens (from peanut Q9SQI9 to peach Q8GT39).

Citation: Tao AL, He SH. Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration. World J Gastroenterol 2004; 10(14): 2103-2108
URL: https://www.wjgnet.com/1007-9327/full/v10/i14/2103.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i14.2103