Contribution of C3d-P28 repeats to enhancement of immune responses against HBV-preS2/S induced by gene immunization

Figure 5 Determination of avidity of anti-HBs IgG of sera from immunized mice. Mice (six mouse per group) were primed im at week 0 with 100 μg of different plasmid DNAs and then boosted sc at week 12 with HBsAg (1 μg per mouse). Sera were obtained at week 18. The pooled serum samples from each mouse group were diluted 1:10 for determination of avidity of the anti-HBs IgG by an HBsAg-specific NaSCN-displacement ELISA. Data were represented as the average of three inde-pendent experiments plus standard errors (mean ± SD).