Prokaryotic expression and renaturation of engineering chimeric Fab antibody against human hepatoma

Figure 5 Relationship between the starting total protein con-centration and denaturation analyzed by SDS-PAGE. 1: High molecular mass marker; 2, 3, 4: Precipitated proteins under reduced condition at starting total protein concentrations of 100, 200, 400 µg/mL, respectively; 5, 6, 7: Precipitated proteins under unreduced condition at starting total protein concentra-tions of 100, 200, 400 µg/mL, respectively.