Expression of cancer-testis antigens in hepatocellular carcinoma

Table 1 PCR amplification program

Gene	Temperature, duration of denaturation, annealing, extension	Primer sequence	Product size (bp)
MAGE-1	94 °C, 45 s	f: 5’-CGGCCGAAGGAACCTGACCCAG-3’	421
	65 °C, 45 s	r: 5’-GCTGGAACCCTCACTGGGTTGCC-3’
	72 °C, 45 s
SSX-1	94 °C, 60 s	f: 5’-CTAAAGCATCAGAGAAGAGAAGC-3’	422
	57 °C, 60 s	r: 5’-AGATCTCTTATTAATCTTCTCAGAAA- 3’
	72 °C, 60 s
CTp11	94 °C, 45 s	f: 5’-CTGCCCCAGACATTGAAGAA-3’	297
	57 °C, 60 s	r: 5’-TCCATGAATTCCTCCTCCTC-3’
	72 °C, 90 s
HCA587	94 °C, 30 s	f: 5’-AGGCGCGAATCA AGTTAG-3’	238
	60 °C, 30 s	r: 5’-CTCCTCTGCTGTGCTGAC-3’
	72 °C, 30 s
Β2-MG	94 °C, 45 s	f: 5’-CTCGCGCTACTCTCTCTTTCTGG -3’	335
	55 °C, 45 s	r: 5’-GCTTACATGTCTCGATCCCACTTA-3’
	72 °C, 45 s

f: Forward primer, r: Reverse primers.