Isolation of murine hepatic lymphocytes using mechanical dissection for phenotypic and functional analysis of NK1.1+ cells

Figure 3 Hepatic lymphocytes isolated with mechanical dissection were suitable for phenotypic analysis of NK1. 1⁺ cells. A: Lymphocytes from liver, lung, spleen and thymus of normal C57BL/6 mice were labeled with two-color immunofluorescence; B, C: C57BL/6 mice were injected with PBS, Poly (I:C) or ConA, respectively. Hepatic lymphocytes were isolated and labeled for phenotype (CD3 and NK1.1) and intracellular cytokine (IFN-γ and TNF-α) detection.