Increased hepatic expression of nitric oxide synthase type II in cirrhotic rats

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Jul 1, 2004 (publication date) through Nov 30, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 1, 2004; 10(13): 1923-1927
Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1923

Table 1 Oligonucleotides used for PCR

Gene	Quantification method	Primer sequence	cDNA
NOS II	Forward primer	5’-CCCTTCCGAAGTTTCTGGCAGCAG-3’	2793-2817
	Reverse primer	5’-GGGCTCCTCCAAGGTGTTGCCC-3’	3424-3446
	Probe	5’-(FAM)TCTTCGGTGCGGTCTTTTCCTATGGAGCAA(TAMRA)-3’	3391-3421
NOS III	Forward primer	5’-CAAGACCGATTACACGACAT-3’	31-51
	Reverse primer	5’-GCTGGTTGCCATAGTGACAT-3’	300-321
	Probe	5’-(FAM)GAGTGTTTGGACAAGTCCTCACCGCCTTTT-(TAMRA)-3’	158-188
GAPDH	Forward primer	5’-GAAGGTGAAGGTCGGAGTCA-3’
	Reverse primer	5’-GAAGATGGTGATGGGA-3’
	Probe	5’-(JOE)CAAGCTTCCCGTTCTCAGCC(TAMRA)-3’

Citation: Wang H, Chen XP, Qiu FZ. Increased hepatic expression of nitric oxide synthase type II in cirrhotic rats. World J Gastroenterol 2004; 10(13): 1923-1927
URL: https://www.wjgnet.com/1007-9327/full/v10/i13/1923.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i13.1923