Quantitative detection of common deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia

Figure 2 Detection results of CD-mtDNA in HCC. A: Conven-tional PCR results: Lane M, marker, Lane 1, negative control, Lane 2, positive control, Lane 3-4, nasopharygitis samples, and Lane 5-8, NPC samples; B: Real-time PCR result shows the amplification plot of fluorescence intensity against the PCR cycle. Each plot corresponds to a HCC sample. The X axis denotes the cycle number of a quantitative PCR reaction. The Y axis denotes the Rn, which is the fluorescence intensity over the background. The correlation coefficient is 0.994.