A rapid and efficient method to express target genes in mammalian cells by baculovirus

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Jun 1, 2004 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 1, 2004; 10(11): 1612-1618
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1612

Table 1 Comparison of gene-transfer and expression efficiency in HepG2 and CV1 cells among different gene-transfer systems

	Gene-transfer rate (%)		Mean fluorescence intensity
	HepG2	CV1	HepG2	CV1
LipofectAMINE	29.6	34.2	138.1	103.4
Retro-EGFP	13.5	18.2	95.8	76.6
Vaccinia-EGFP	93.5^a	94.2^a	172.5^a	148.4¹
BacV-CMV-EGFPA	92.5	95.6	294.5	232.1

¹Cytopathic effects could be observed within 24 h post infec-tion and most cells were lysised within 48 h.

Citation: Cheng T, Xu CY, Wang YB, Chen M, Wu T, Zhang J, Xia NS. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol 2004; 10(11): 1612-1618
URL: https://www.wjgnet.com/1007-9327/full/v10/i11/1612.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i11.1612