A rapid and efficient method to express target genes in mammalian cells by baculovirus

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 10, Issue 11

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

All Articles published online

Item

Count

PDF

594

HTML

3461

Figures (1-6)

250

Tables (1-3)

148

Sum=4453

Jun 1, 2004 (publication date) through Aug 24, 2024

Times Cited of This Article

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Research

World J Gastroenterol. Jun 1, 2004; 10(11): 1612-1618
Published online Jun 1, 2004. doi: 10.3748/wjg.v10.i11.1612

Table 1 Comparison of gene-transfer and expression efficiency in HepG2 and CV1 cells among different gene-transfer systems

	Gene-transfer rate (%)		Mean fluorescence intensity
	HepG2	CV1	HepG2	CV1
LipofectAMINE	29.6	34.2	138.1	103.4
Retro-EGFP	13.5	18.2	95.8	76.6
Vaccinia-EGFP	93.5^a	94.2^a	172.5^a	148.4¹
BacV-CMV-EGFPA	92.5	95.6	294.5	232.1

¹Cytopathic effects could be observed within 24 h post infec-tion and most cells were lysised within 48 h.

Citation: Cheng T, Xu CY, Wang YB, Chen M, Wu T, Zhang J, Xia NS. A rapid and efficient method to express target genes in mammalian cells by baculovirus. World J Gastroenterol 2004; 10(11): 1612-1618
URL: https://www.wjgnet.com/1007-9327/full/v10/i11/1612.htm
DOI: https://dx.doi.org/10.3748/wjg.v10.i11.1612