Association of differentially expressed genes with activation of mouse hepatic stellate cells by high-density cDNA mircoarray

Table 3 A portion of up-regulated genes in activated HSC

GenBank accession number	Gene name	Ratio (Cy5/Cy3)	Potential gene function
AF061017	UDP-glucose dehydrogenase, mRNA complete cds	15.56	Glycosaminoglycan , hyaluronan and heparin sulfate biosynthesis
X65553	mRNA for poly (A) binding protein	7.405	A regulator of translation initiation
AF029844	Elongation factor 1-beta homology mRNA, complete cds	6.305	Translation factor and has multi-functions
NM_010798	Macrophage migration inhibitory factor (Mif), mRNA	5.847	Proinflammatory peptide and a mediator of growth factor dependent ERK MAP kinase activation and cell cycle progression
M31131	Neural cadherin (N-cadherin) mRNA	5.559	Cell adhesion molecule
AB029930	mRNA for caveolin-1 beta isoform	4.945	Inhibit the eNOS activity
D12618	mRNA for nucleosome assembly protein-1, complete cds	4.528	Histone H2A-H2B shutting protein that promotes histone deposition, and is important for maintaining chromatin structure
M22432	Protein synthesis elongation factor Tu (eEF-Yu, eEf-1-1alpha) mRNA, complete cds	4.344	Translation factor involved in the protein biosynthesis
D31717	mRNA for ribophorin	4.319	Glycoprotein synthesis
NM_012052	Ribosomal protein S3 (Rps3), mRNA	4.297	Protein biosynthesis
X72711	mRNA for replication factor C (140 Kda), long subunit	4.245	DNA synthesis and repair, and a regulator of NF-kappa B
X13460	Annexin VI, p68 (Mouse mRNA for p68 protein of the lipocortin family)	4.218	Calcium binding protein
NM_011290	Ribosomal protein L6 (Rp16), mRNA	4.089	Protein biosynthesis
U12273	Apurinic/apyrimidinic endonuclease (APEX) gene, complete cds	3.488	A DNA repair enzyme and an activator of several transcription factors
D42048	mRNA for squalene epoxidase	3.298	Rate-limiting enzyme of cholesterol biosynthesis
AJ250491	mRNA for receptor activity modifying protein 3 (Ramp3) gene	3.255	Complex with the calcitonin receptor-like receptor, establishing a functional receptor for adrenomedullin
L04280	Ribosomal protein (RPL12) mRNA	3.172	Protein biosynthesis
K02927	Ribonucleotide reductase subunit M1 mRNA, complete cds	3.156	Rate-limiting enzyme in DNA synthesis and repair
AF087568	Palmitoyl-protein thioesterase precursor mRNA, complete cds	3.059	Cell survival signaling
AF020039	NADP-dependent isocitrate dehydrogenase (Idh) mRNA, complete cds	3.038	A key enzyme of the tricarboxylic acid cycle
X57960	Ribosomal protein L7	2.962	Protein biosynthesis
U09816	GM2 activator protein (Gm2a) mRNA	2.888	Enzymatic hydrolysis of GM2
AB018575	Cdc 7 mRNA, complete cds	2.816	Essential for G1/S transition
AF041054	E1B19K/Bcl-2 binding protein homology (Nip3) mRNA, nuclear gene encoding mitochondria protein, complete cds	2.813	Nips is a pro-apoptotic mitochondria protein
L04128	Ribosomal protein L18 (rpL18) mRNA	2.751	Protein biosynthesis
Z31554	(129/SV) Cctd mRNA for CCT (chaperonin containing TCP-1) delta subunit	2.743	Stabilize the cytoskeletal protein actin and tubulin
D63784	mRNA for MIDA1, complete cds	2.703	Regulate cell growth
AF026481	eIF-1A (eIF-1A) mRNA, complete cds	2.682	Translation initiation factor
U35249	CDK-activating kinase assembly factor p36/MAT1, complete cds	2.645	An assembly factor and a targeting subunit of cyclin-dependent kinase-activating kinase that is involved in cell cycle control, transcription and DNA repair
M76131	Elongation factor (ef-2) mRNA, 3’end	2.614	Protein biosynthesis
X64713	mRNA for cyclin B1	2.576	Cell cycle regulatory protein
D26091	mRNA for mCDC47, complete cds	2.570	A member of the minichromosome maintenance (Mcm) family involve in the DNA replication licensing system
U78085	Ribosomal protein S5 mRNA	2.507	Protein synthesis
AF141322	Caveolin-2 mRNA, complete cds	2.458	Inhibit the eNOS activity
M33934	IMP dehydrogenase mRNA, complete cds	2.429	An enzyme involved in de novo synthesis of guanine nucleotides
U83628	Defender against cell death protein 1 (DAD1) mRNA, complete cds	2.372	Apoptotic suppressor gene