Polymorphisms at cholesterol 7α-hydroxylase, apolipoproteins B and E and low density lipoprotein receptor genes in patients with gallbladder stone disease

Figure 1 Genotypes of CYP7A, apolipoproteins B, E and LDL receptor gene. A: CYP7A gene, M: DNA Marker: 1000, 750, 500, 300, 150 bp (Cat. G3161, Promega, Medison, WI, USA); lanes 1, 2: AA type; lanes 3-5: AC type; lane 6: CC type; lanes 7, 8: PCR products, B: Apolipoprotein B gene, M: DNA Marker: 1543, 994, 695, 515, 377, 237 bp (Cat. MG0781, SABC, Shanghai, China), lane 1, 3, 5, 6: X+/- type: 2, 4, 7: X-/- type. C: Apo E gene, M: pGEM 7zf(+)/Hae III DNA Marker (Cat. MG0861, SABC, Shanghai, China), from lanes 1-9: ε3/3, ε2/2, ε3/4, ε2/4, ε2/3, ε4/4, ε3/4, ε2/2, ε3/3 types. D: LDL receptor gene, M: pGEM 7zf (+)/Hae III DNA Marker, from lanes 1-6: CC, BC, AA, AC, BC and AB types.