Transcriptional regulation of human α1(I) procollagen gene in dermal fibroblasts

Figure 3 Electrophoresis (20 g/L agarose gel) of the fragment -268 to +42 bp digested with EcoR II. Lane1: EcoR II-digested fragment, Lane2: 100 bp DNA ladder, Lane3: 310 bp length of fragment spanning from -268 to +42 bp. The 310 bp fragment spanning -268 to +42 bp of the human α1(I) procollagen gene was obtained by PCR with p5.3Kα1 as template. The fragment was digested with EcoR II. Electrophoresis (20 g/L agarose gel) of the digested mixture showed 3 bands with different sizes (42 bp, 113 bp, 155 bp) which were labeled and used as probes in EMSA.