Transcriptional regulation of human α1(I) procollagen gene in dermal fibroblasts

Figure 2 Effects of consensus DNA on CAT expression in pCOLH₁0. 27 transfected cells. Control: mock DNA transfection, Ap-1: transfection of Ap-1 consensus DNA (10 μg), Sp-1: trans-fection of Sp-1 consensus DNA (10 μg), NF-1: transfection of NF-1 consensus DNA (10 μg). For the cells transfected with pCOLH₁0.27, transfection of consensus recognition DNA for Ap-1, Sp-1 or NF-1 was performed 24 h after initial transfection. Reporter gene CAT was determined with ELISA after another 24 h. Relative CAT values in different transfection groups were calculated relative to that in mock DNA transfection. The result represented three independent experiments. ^aP < 0.05 compared to control.