Basic Research
Copyright ©The Author(s) 2004.
World J Gastroenterol. May 15, 2004; 10(10): 1447-1451
Published online May 15, 2004. doi: 10.3748/wjg.v10.i10.1447
Figure 1
Figure 1 Electrophoresis of six constructs digested with Mlu I and Xho I. M1: 100 bp DNA ladder marker, M2: 1 kb DNA ladder marker, Lane1: pCOLH10.1, lane2: pCOLH10.27, lane3: pCOLH10.5, Lane4: pCOLH10.9, Lane5: pCOLH11.5, Lane6: pCOLH12.5. Six recombinant plasmids containing serial 5’-de-leted flanking sequences of human α1(I) procollagen gene as putative promoter were digested with Mlu I and Xho I at 37 °C for 1 h. The digested DNAs were fractionated on 15 g/L agar-ose gel showing vector DNA (4.3 kb) and insertion promoters with different sizes.