Transcriptional regulation of human α1(I) procollagen gene in dermal fibroblasts

Figure 1 Electrophoresis of six constructs digested with Mlu I and Xho I. M1: 100 bp DNA ladder marker, M2: 1 kb DNA ladder marker, Lane1: pCOLH₁0.1, lane2: pCOLH₁0.27, lane3: pCOLH₁0.5, Lane4: pCOLH₁0.9, Lane5: pCOLH₁1.5, Lane6: pCOLH₁2.5. Six recombinant plasmids containing serial 5’-de-leted flanking sequences of human α1(I) procollagen gene as putative promoter were digested with Mlu I and Xho I at 37 °C for 1 h. The digested DNAs were fractionated on 15 g/L agar-ose gel showing vector DNA (4.3 kb) and insertion promoters with different sizes.