Differential expression of cholangiocyte and ileal bile acid transporters following bile acid supplementation and depletion

Figure 4 Effect of TCA and CY feeding on the amount of ASBT and t-ASBT protein in ileum. Bar graphs showing ratios of ASBT/β-actin and t-ASBT/β-Actin in PNS of ileal scrapings obtained from rats fed C, TCA and CY diets. Forty and 80 μg of protein were subjected to SDS-PAGE and immunoblotted to visualize ASBT and t-ASBT, respectively. Data shown here are representative of n = 3 feeding studies were analyzed per group and expressed as mean ± SE (^aP < 0.05). For the analysis of ASBT and t-ASBT in the TCA treated groups, 7 and 3 rats in total were used, respectively. Whereas, the number of animals ana-lyzed in the CY groups totaled 10 and 3 for ASBT and t-ASBT, respectively. Hence, the number of control rats was 17 for ASBT and 6 for t-ASBT in total.