Alterations in expression, proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma

Figure 3 Western blot analysis of expression of multiple forms of clusterin in ESCC. nCLU: the nonglycosylated uncleaved nuclear clusterin; α/β CLU: cleaved and glycosylated secreted clusterin protein; β CLU: cleaved and glycosylated clusterin β chain; B-5: monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) reacted with the C-terminus of clusterin-β of human origin and did not react with the nuclear clusterin; H-330: polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA) recognized the major part of clusterin from 120 to 449 aa at the C-terminus of clusterin-α/β chains including unleaved nuclear clusterin (-50 ku and the matured secreted clusterin (-37-40 ku); Np: pooled normal esophageal mucosa from 10 cases; Cp: pooled ESCC tumor tissues from 10 cases; N109: normal esophageal mucosa from a single individual; C109: ESCC tumor tissue from a single individual. β-actin was used as loading control.