Alterations in expression, proteolysis and intracellular localizations of clusterin in esophageal squamous cell carcinoma

Figure 1 Schematic diagram of analyzed fragments of clusterin. A: structural map of the human clusterin protein. Letters and boxes signify the following: ER-targeting signal, endoplasmic reticulum-targeting hydrophobic leader peptide; splice site, α/β-cleavage site (amino acid 205 and 206) of cytoplasmic - 60 ku pre-secreted clusterin which was glycosylated and cleavaged into α and β chains to form an 80 ku matured secre-tory clusterin protein; rectangles, hydrophobic leader peptide (N-terminal 66 bp encode aa M1 to G22, 22 aa), β-chain (5’ end) and α-chain (3’ end); Red letters, the glycosylation sites. B: Schematic diagram of analyzed fragments of clusterin and their RT-PCR results.