Polymorphisms at cholesterol 7α-hydroxylase, apolipoproteins B and E and low density lipoprotein receptor genes in patients with gallbladder stone disease

Table 1 PCR condition and restriction enzymes

Genes	Primers	Annealing temp	RE
CYP7A[16,17]	5’TGGTAGGTAAATTATTAATAGATGT3’
	5’AAATTAAATGGATGAATCAAAGAGC3’	61°C	Bsa I
apo B[10]	5’GGAGACTATTCAGAAGCTAA3’
	5’GAAGAGCCTGAAGACTGACT3’	60°C	Xba I
apo E[24]	5’ACAGAATTCGCCCCGGCCTGGTACAC3’
	5’TAAGCTTGGCACGGCTGTCCAAGGA3’	60°C	Hha I
LDL receptor[25]	5’CACTTTGTATATTGGTTGAAACTGT3’
	5’CACTGAACAAATACAGCAACCAGGG3’	62°C