Construction of human liver cancer vascular endothelium cDNA expression library and screening of the endothelium-associated antigen genes

Table 2 Construction of HLCVECs libraries in λ-ZAP expression vector and the number of SEREX-identified antigens

Patient	Age (years)	Primary size	Screening serum	Clones screened	Positive clones	cDNA fragment	Known genes	Unknown genes
HCC	56	2 × 106	immunized	6 × 105	36	18	15	3