Superantigen-SEA gene modified tumor vaccine for hepatocellular carcinoma: An in vitro study

Figure 7 Membrane expressed SEA enhanced specific lysis on autologous tumor cells. Cytotoxicity was measured in a stan-dard 4-hour ⁵¹Cr-release assay. Target cells (HepG2, SMMC-7721) were seeded in triplicate in v-bottomed microtiter plates at a concentration of 5 × 10³ cells per well. Effector cells were added at an effector-to-target (E:T) cell ratio of 40:1, 25:1, 10:1, 5:1 and 3:1 respectively. A: SEA-T was highly efficient in di-recting specific lysis of HepG2 cells (squares), HepG2-T has moderate cytotoxicity on HepG2 (cross) cells (P < 0.001). B: None of T cell line has killing effect on SMMC-7721 cells.