Transfection of mEpo gene to intestinal epithelium in vivo mediated by oral delivery of chitosan-DNA nanoparticles

Figure 5 mEpo expression and its physiological effect test. He-matocrit was measured every two days in mice fed with (●) chitosan-mEpo and doxycycline (200 µg/mL), n = 9; (▲) chitosan-mEpo alone, n = 3; (◆) doxycycline (200 µg/mL) alone, n = 4; (■) naked mEpo and doxycycline (200 µg/mL), n = 5.↓indicating the mice fed with ‘naked’ mEpo DNA or chitosan-Epo.