Loss of heterozygosity at adenomatous polyposis coli, mutation in colorectal cancer and deleted in colorectal cancer genetic loci in colorectal cancers

Table 1 Primer sets used in polymerase chain reaction-loss of heterozygosity assays

Primer set1	Priming region	Amplification size (base pairs)	Polymorphism type	Primer sequence
1	APC exon 11	133	Rsal RFLP	5'-GGACTACAGGCCATTGCAGAA-3'
				5'-GGCTACATCTCCAAAAGTCAA-3'
2	MCC exon 10	79 or 93	Insertion	5'-TACGAATCCAATGCCACA-3'
				5'-CTGAAGTAGCTCCAAACA-3'
3	DCC	396	MspI RFLP	5'-TTGCACCATGCTGAAGATTGT-3'
				5'-ACCCTCCCCCTGATGACTTA-3'
4	DCC	240	MspI RFLP	5'-CGACTCGATCCTACAAAATC-3'
				5'-TCTACCCAGGTCTCAGAG-3'
5	DCC	200	VNTR	5'-GATGACATTTTCCCTCTAG-3'
				5'-GTGGTTATTGCCTTGAAAAG-3'

¹Primer sets 1 and 2, Tumura et al[6]; Primer sets 3, 4, and 5, Gao et al[5]. VNTR: Variable number of tandem repeats; APC: Adenomatous polyposis coli; MCC: Mutation in colorectal cancer; DCC: Deleted in colorectal cancer; RFLP: Restriction fragment length polymorphism analysis technique; RFLP: Restriction fragment length polymorphism.