Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches

Figure 1 2-DE profiles of the pre- and post-surgery matched esophageal cancer sera from a same individual. One hundred and eighty microgram of protein was separated by 2-DE (IEF at pH3-10, 13% of SDS-PAGE) and stained by silver staining. LMW markers were from Amersham Pharmacia. (A) pre-sur-gery serum, (B) matched post-surgery serum.

Figure 2 Representative regions of 2-DE patterns of more matched esophageal cancer sera. One hundred and eighty microgram of protein was separated by 2-DE (IEF at pH3-10, SDS-PAGE 13%) and stained by silver staining. LMW mark-ers were from Amersham Pharmacia. (A) pre-surgery sera vs post-surgery sera, (B) pre-surgery sera vs normal control sera.

Figure 3 The spectra of MALDI-TOF-MS obtained from one of the differentiated polypeptide spots matched with the tryptic peptide sequences of clusterin (characters in red).

Figure 4 (A) Western blot analysis of clusterin proteins expressed in patient-matched normal and tumor epithelium; (B) Western blot analysis of clusterin proteins expressed in two kinds of esophageal squamous cell carcinoma, EC0156 and EC0132. Alpha-tubulin was used as a loading control. (C) Expression analysis of clusterin in patient-matched esophageal cancer tissues by semi-quantitative RT-PCR. GAPDH was used as an internal control.