The point mutation of p53 gene exon7 in hepatocellular carcinoma from Anhui Province, a non HCC prevalent area in China

Figure 1 Agarose gel electrophoresis of PCR products of p53 exon7. A: 1-6. PCR products of p53 exon7 amplified from HCC genomic DNA 7.100 bp DNA ladder; B: 1-6. PCR products of CDK4 gene amplified from HCC genomic DNA as control

Figure 2 PCR-SSCP of p53 exon7 Samples of 1, 6, 9 have point mutations of p53 exon7 Samples of 2, 3, 4, 5, 7, 8 don’t have point mutations of p53 exon7

Figure 3 PCR-RFLP of p53 codon249. 1. 100 bp DNA ladder; 2 and 4: heterozygous point mutation of p53 codon249; 3, 5, 6 and 7: p53 Exon7 wild type.

Figure 4 Sequencing of p53 exon 7 PCR product

Figure 5 Comparison between the sequencing result of PCR products of p53 exon7 and that published by the Genbank. [Capital letters shows that A (mutation) takes place of C (wild type)]