Construction of retroviral vectors to induce a strong expression of human class interferon gene in human hepatocellular carcinoma cells in vitro

Figure 1 Structure of MNSM, MNSIFNB, MNAIFNB, MNSIFNA, MNAIFNA, and AMNSIFNA retroviral vectors. LTR: Retroviral long-terminal repeat sequence; Neo R: Neomycin phosphotransferase gene for G418 selection; SV40: Simian virus 40 early region promoter; HuIFN-β-cDNA: Human interferon β complementary DNA; HuIFN-α-cDNA, Human interferon α complementary DNA. AFPe: Human α-fetoprotein “house-keeping gene” enh ancer; AFPe.c, Human α-fetoprotein “house-keeping gene” enhancer core sequence.

Figure 2 Northern blot of total RNA from the tumor cells modified with human interferon-α gene, probed with ³²P labeled human interferon-α cDNA. Lane 1: Unmodified HepG2 cells; Lane 2: MNSM modified HepG2 cells; Lane 3: MNSIFNA modified HepG2 cells; Lane 4: MNAIFNA modified HepG2 cells; Lane 5: MNSIFNB modified HepG2 cells; Lane 6: MNSIFNA modified HepG2 cells; Lane 7: AMNSIFNA modified HepG2 cells; Lane 8: MNAIFNB modified HepG2 cells.

Figure 3 Dot blot of total RNA from human tumor cells modified with HuIFN-β gene, and fully selected with G418, probed with 32P labeled HuIFN-β-cDNA. Lane 1: Hep3B cells infected with MNAIFNB retroviruses; Lane 2: Hep3B cells infected with MNSIFNB retroviruses; Lane 3: HepG2 cells infected with MNAIFNB retroviruses; Lane 4: HepG2 cells infected with MNSIFNB retroviruses; Lane 5: M21 cells infected with MNSIFNB retroviruses.