lncRNA-SNHG15 accelerates the development of hepatocellular carcinoma by targeting miR-490-3p/ histone deacetylase 2 axis

Figure 1 The dysregulation of lncRNA-SNHG15 in hepatocellular carcinoma. A: SNHG15 expression in 33 randomly selected hepatocellular carcinoma tissues and normal tissues; B: SNHG15 expression in HuH-1, HuH-7 and L-O2 cell lines; C: lncRNA-SNHG15 expression in HuH-1 and L-O2 cells containing SNHG15 siRNA or vector; D: Cell proliferation in HuH-1 and L-O2 cells with SNHG15 siRNA or vector; E: Cell migration in HuH-1 cells with SNHG15 siRNA or vector; F: Cell invasion in HuH-1 cells with SNHG15 siRNA or vector. Untreated cells were used as control (NC). ^aP < 0.05, ^bP < 0.01. SNHG15: Small nucleolar RNA host gene 15; HuH-1: Hepatocellular carcinoma cell line; HuH-7: Hepatocellular carcinoma cell line; L-O2: Normal human liver cell lin3; siRNA: Small interfering RNA.

Figure 2 Direct interaction of lncRNA-SNHG15 and miR-490-3p. A: The binding sites between lncRNA-SNHG15 with miR-490-3p; B: Luciferase reporter assay; C: miR-490-3p expression in 33 randomly selected HCC tissues and normal tissues; D: SNHG15 had negative correlation with miR-490-3p expression in 33 randomly selected HCC tissues; E: miR-490-3p expression regulated by SNHG15 siRNA or vector in HuH-1 cells; F: SNHG15 expression in HuH-1 cells containing miR-490-3p mimics or inhibitor; G: miR-490-3p expression in cells with miR-490-3p mimics or miR-490-3p mimics + SNHG15 vector. H: Cell proliferation in HuH-1 cells containing miR-490-3p mimics or miR-490-3p mimics + SNHG15 vector; I: Cell migration in HuH-1 cells containing miR-490-3p mimics or miR-490-3p mimics + SNHG15 vector; J: Cell invasion in HuH-1 cells containing miR-490-3p mimics or miR-490-3p mimics + SNHG15 vector. Untreated cells were used as control (NC). ^bP < 0.01. SNHG15: Small nucleolar RNA host gene 15; miR-490-3p: Hsa-miR-490-3p; HCC: Hepatocellular carcinoma; siRNA: Small interfering RNA; HuH-1: Hepatocellular carcinoma cell line.

Figure 3 miR-490-3p directly targets HDAC2. A: The binding sites between miR-490-3p and HDAC2; B: Luciferase reporter assay; C: miR-490-3p regulated HDAC2 expression in HuH-1 cells; D: miR-490-3p regulated HDAC2 expression in HuH-1 cells; E: HDAC2 expression in 33 randomly selected hepatocellular carcinoma tissues and normal tissues; F: HDAC2 was negatively correlated with miR-490-3p in 33 randomly selected HCC tissues; G: lncRNA-SNHG15 was positively correlated with HDAC2 in 33 randomly selected HCC tissues. Untreated cells were used as control (NC). ^bP < 0.01. miR-490-3p: Hsa-miR-490-3p; HDAC2: Histone deacetylase 2; HuH-1: Hepatocellular carcinoma cell line; HCC: Hepatocellular carcinoma; SNHG15: Small nucleolar RNA host gene 15.

Figure 4 HDAC2 regulated hepatocellular carcinoma progression through mediating SNHG15/miR-490-3p. A: HDAC2 expression in HuH-1 cells containing HDAC2 siRNA, si-HDAC2 + miR-490-3p inhibitor or si-HDAC2 + SNHG15 vector; B: HDAC2 expression in HuH-1 cells containing HDAC2 siRNA, si-HDAC2 + miR-490-3p inhibitor or si-HDAC2 + SNHG15 vector; C: Cell proliferation in HuH-1 cells containing HDAC2 siRNA, si-HDAC2 + miR-490-3p inhibitor or si-HDAC2 + SNHG15 vector; D: Cell migration in HuH-1 cells containing HDAC2 siRNA, si-HDAC2 + miR-490-3p inhibitor or si-HDAC2 + SNHG15 vector; E: Cell invasion in HuH-1 cells containing HDAC2 siRNA, si-HDAC2 + miR-490-3p inhibitor or si-HDAC2 + SNHG15 vector. Untreated cells were set as control (NC). ^bP < 0.01. SNHG15: Small nucleolar RNA host gene 15; miR-490-3p: Hsa-miR-490-3p; HDAC2: Histone deacetylase 2; HuH-1: Human hepatoma cell line; siRNA: Small interfering RNA; si-HDAC2: HDAC2 siRNA.