Immunolocalization of nestin in pancreatic tissue of mice at different ages

Figure 1 Immunolocalization of nestin in mice pancreases at different ages. A: Immunohistochemical localization of Nestin (red), Insulin (green), glucagon (blue) in three-day-old mouse pancreatic section, the presence of nestin is more towards the periphery of the endocrine fraction, magnification (× 400); B: One-week-old mouse pancreatic section, the presence of nestin is more towards the periphery of the endocrine fraction, magnification (× 400); C: Two-week-old mouse pancreatic section showing Nestin staining in both the exocrine as well as endocrine fraction, magnification (× 400); D: Four-week-old mouse pancreatic section showing Nestin staining only in the endocrine fraction confining more towards the periphery of the insulin stained cells, magnification (× 400); E: Eight-week-old mouse pancreatic section showing the Nestin localization predominant in the central region of the islets clusters where immunostaining for insulin was also significant, magnification (× 400).

Figure 2 Quantitative relative fluorescence units of Nestin for different age groups performed in three different sections showing a higher level in the 8-wk-old pancreatic tissue. ^aP < 0.05 in the adult as as compared to other four ages.