Inhibition of hepatitis B virus replication by pokeweed antiviral protein in vitro

Figure 1 Effects of PAP-S on secreted HBsAg (A), HBeAg (B) and HBV DNA(C) in HepG2 2. 2.15 cell cultures. Data are expressed as mean ± SD of three independent experiments. Except the first group (0.1 &mgr;g/mL), P < 0.01 vs the corresponding controls (Student’s t-test).

Figure 2 Western blot analysis of the expression of pXF3H-PAP in cotransfected HepG2 cells β-actin as internal reference; 1: pHBV1. 3 (1.0 &mgr;g) + pXF3H (2.0 &mgr;g); 2: pHBV1.3 (1.0 &mgr;g) + pXF3H-PAP (2.0 &mgr;g); 3: pHBV1.3 (1.0 &mgr;g) + pXF3H-PAP (1.0 &mgr;g).

Figure 3 Southern blot analysis of the effect of pXF3H-PAP on intracellular HBV DNA replication in cotransfected HepG2 cells 3 d after transfection. RC: Relaxed circular DNA; SS: Single stranded DNA. 1: pHBV1.3 (1.0 &mgr;g) + pXF3H (2.0 &mgr;g) 2:pHBV1.3 (1.0 &mgr;g) + pXF3H-PAP (1.0 &mgr;g); 3: pHBV1.3 (1.0 &mgr;g) + pXF3H-PAP (2.0 &mgr;g); 4. pHBV1.3 (1.0 &mgr;g) + 3TC (20.0 &mgr;mol/L).

Figure 4 Northern blot analysis of the effect of pXF3H-PAP on intracellular HBV RNA transcription in cotransfected HepG2 cells on d 3 after transfection. GAPDH as internal reference; 1: pHBV1.3 (1.0 &mgr;g) + pXF3H (2.0 &mgr;g) 2: pHBV1.3 (1.0 &mgr;g) + pXF3H-PAP (2.0 &mgr;g).