Regulatory T cells in patients with inflammatory bowel diseases treated with adacolumn granulocytapheresis

Figure 1 Variation of peripheral blood CD4+ CD25+ cells in patients treated by GMA-apheresis. A: Representative cytogram; B: Variation of T regs on each individual patient; C: Variation in the ratio CD4+ CD25^high/CD4+ CD25^low-int.

Figure 2 Variation of mRNA expression for FoxP3 in CD4+ T cells in matched samples from 6 patients treated by GMA-apheresis: Quantitative PCR was performed as described in methods; Results are expressed as FoxP3 expression relative to GUS expression per cent.

Figure 3 Variation of mRNA expression for TGF-beta in CD4+ T cells in matched samples from 6 patients treated by GMA-apheresis: Quantitative PCR was performed as described in methods; Results are expressed as TGF-beta 1 expression relative to GAPDH expression per cent.

Figure 4 Improvement of pyode-rma gangrenosum in a patient with Crhon’ disease treated by GMA-aphersesis: pictures A-D, were taken at 1, 5, 8, and 13 apheresis sessions.