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World J Gastroenterol. Dec 28, 2007; 13(48): 6568-6574
Published online Dec 28, 2007. doi: 10.3748/wjg.v13.i48.6568
Published online Dec 28, 2007. doi: 10.3748/wjg.v13.i48.6568
Figure 1 Standard DNA template with 10 fold serial dilutions used to develop the standard curve.
1: 9 ×108 copies/μL; 2: 9 × 107 copies/μL; 3: 9 × 106 copies/μL; 4: 9 × 105 copies/μL; 5: 9 × 104 copies/μL; 6: 9 × 103 copies/μL.
Figure 2 A: Sensitivity of FQ-PCR detection using 10 fold serial diluted S.
enteritidis (Mouse, 50 338) standard template. 1: 4.0 × 103 copies/μL; 2: 4.0 × 102 copies/μL; 3: 4.0 × 101 copies/μL; 4: 4.0 × 100 copies/μL; 5: 4.0 × 10-1 copies/μL; B: Sensitivity of FQ-PCR detection used 10 fold serial diluted S. Enteritidis (Mouse, 50338) cell number. 1: 6.0 × 103 CFU/mL; 2: 6.0 × 102 CFU/mL; 3: 6.0 × 101 CFU/mL; 4: 6.0 × 100 CFU/mL ; 5: 6.0 × 10-1 CFU/mL.
Figure 3 Reproducibility of FQ-PCR.
1: 1.2 × 109 copies/μL; 2: 1.2 × 108 copies/μL; 3: 1.2 × 107 copies/μL ; 4: 1.2 × 106 copies/μL.
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Citation: Deng SX, Cheng AC, Wang MS, Cao P. Gastrointestinal tract distribution of
Salmonella enteritidis in orally infected mice with a species-specific fluorescent quantitative polymerase chain reaction. World J Gastroenterol 2007; 13(48): 6568-6574 - URL: https://www.wjgnet.com/1007-9327/full/v13/i48/6568.htm
- DOI: https://dx.doi.org/10.3748/wjg.v13.i48.6568