Regulation of activin receptor-interacting protein 2 expression in mouse hepatoma Hepa1-6 cells and its relationship with collagen type IV

Figure 1 Expressions of ARIP2 mRNA in Hepal-6 cells stimulated by activin A and LPS.

Figure 2 The effects of signal transduction activators on expressions of ARIP2 mRNA. Lane 1: Hepal-6 cells untreated; Lane 2: Treated with activin A (5 ng/mL); Lane 3: A23187 (200 nmol/L); Lane 4: LPS (2.5 μg /mL); Lane 5: PMA (20 nmol/L); Lane 6: forskolin (50 μmol/L ).

Figure 3 The mRNA expressions of ActRIIA and ActRIIB in ARIP2-overexpressed Hepal-6 cells. Lane 1 and 2: Hepal-6 cells were transfected with empty vector pcDNA3 (0.3 μg); lane 3: pcDNA3-ARIP2 (0.1 μg) + pcDNA3 (0.2 μg); lane 4: pcDNA3-ARIP2 (0.3 μg).

Figure 4 ARIP2-overexpression suppressed the expressions of collagen IV mRNA in Hepal-6 cells. lane 1 and 2: Hepal-6 cells were transfected with empty vector pcDNA3 (0.3 μg); lane 3: pcDNA3-ARIP2 (0.1 μg) + pcDNA3 (0.2 μg); lane 4: d pcDNA3-ARIP2 (0.3 μg). The cells were incubated in the absence (lane 1) or the presence of activin A (5 ng/mL) (lane 2, 3 and 4) for 24 h.

Figure 5 Flow cytometry analysis of collagen IV protein expressions in Hepal-6 cells. A: Hepal-6 cells were transfected with 0.3 μg pcDNA3; B: with 0.3 μg pcDNA3-ARIP2. 16% or 2% expressed the percent of positive fluorescence cells in A or B.