Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Figure 1 Tissue ATP Content as a parameter for functional recovery of the mitochondria and resultant tissue integrity after 18 h cold storage and 30 min oxygenated reperfusion. Data are expressed as micromoles per gram of tissue dry-weight (mean ± SE, n = 6 each). ^aP < 0.05 vs UW, ^bP < 0.001 vs UW, ^cP < 0.05 vs Celsior.

Figure 2 LDH release into the perfusate, as a parameter for general tissue damage after 18 h cold storage and 30 min oxygenated reperfusion (mean ± SE, n = 7 each). ^aP < 0.05 vs UW.

Figure 3 MDA concentrations in the graft tissues, as an index for lipid peroxidation after 18 h cold storage and 30 min oxygenated reperfusion (mean ± SE, n = 6 each). ^bP < 0.001 vs Celsior, HTK and Polysol.

Figure 4 Tissue oxygen consumption at 30 min oxygenated reperfusion, as a parameter for the remaining metabolic activity of the grafts (mean ± SE, n = 7 each). ^bP < 0.01 vs HTK and Celsior, ^dP < 0.001 vs UW.

Figure 5 The release of fragmented DNA into the perfusate at 15 min of reperfusion using a Cell Death Detection ELISA kit (mean ± SE, n = 6 each). ^bP < 0.01 vs HTK, ^dP < 0.001 vs Celsior and Polysol.

Figure 6 A-D: ultrastructural analysis using electron microscopy, microvilli condition after 18 h CS; E-H: ultrastructural analysis using electron microscopy, mitochondria in enterocytes after 18 h CS.