Viral Hepatitis
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Nov 7, 2005; 11(41): 6440-6444
Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6440
Figure 1
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Figure 1 Three-dimensional structure of FHV capsid protein.
Figure 2
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Figure 2 Recombinant proteins expressed in E. coli cells. M: protein molecular mass standard; lane 1: recombinant vector protein Wt; lane 2: chimeric protein I3S; lane 3: L3C1-I2E-L1C2-L2E; lane 4: L3C1-I2E-L1C2-L2E-I3S; lane 5: supernatant of BL21 cells transformed by pET-L3C1-I2E-L1C2-L2E; lane 6: non-transformed BL21 cells.
Figure 3
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Figure 3 Expression of purified expressed proteins. M: protein molecular mass standard; lane 1: recombinant vector protein Wt; lane 2: chimeric protein I3S; lane 3: L3C1-I2E-L1C2-L2E; lane 4: L3C1-I2E-L1C2-L2E-I3S.
Figure 4
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Figure 4 Western blot of expressed proteins using anti-HCV+ (A) and anti-HBsAg+ (B) sera as detecting antibodies. M: protein molecular mass standard; lane 1: recombinant vector protein W; lane 2: chimeric protein I3S, lane 3: L3C1-I2E-L1C2-L2E; lane 4: L3C1-I2E-L1C2-L2E-I3S.