Inhibitory effect of antisense vascular endothelial growth factor 165 eukaryotic expression vector on proliferation of hepatocellular carcinoma cells

Figure 1 Effect of PCDNA3-as-VEGF₁₆₅ on VEGF mRNA ex-pression of hepatocarcinoma SMMC-7721 cells by RT-PCR. VEGF mRNA expression was negative after PCDNA3-as-VEGF₁₆₅/LF2000 transfected. (Lane 1: PCDNA3/LF2000 transfected, Lane 2: PCDNA3-as-VEGF165/LF2000 transfected; β-actin: 587 bp, VEGF 258 bp).

Figure 2 Immunohistochemical staining for expression of VEGF protein after DNA/LF2000 transfection in hepatocarcinoma SMMC-7721 cells. Positive signal for VEGF protein is brown and located in cytoplasm. A: (×100), B: (×400): PCDNA3 transferred SMMC-7721 cells; C: (×100), D: (×400): PCDNA3-as-VEGF165 transferred.

Figure 3 Effect of PCDNA₃-as-VEGF₁₆₅ on proliferation of hepatocarcinoma SMMC-7721 cells by MTT.