Genetic alterations and reduced expression of tumor suppressor p33ING1b in human exocrine pancreatic carcinoma

Figure 1 Representative examples of IHC staining for p33^ING1b (× 200) on normal pancreatic tissues and cancer tissues. A: Weak positive pancreatic cells for p33^ING1b; B: No p33^ING1b; C and D: Extremely strong staining in pancreatic cancer samples.

Figure 2 SSCP analysis of p33^ING1b gene in genomic DNA from pancreatic carcinomas. N: normal DNA; T: tumor DNA. Arrow point indicates the altered band in tumor DNA as compared with the corresponding normal DNA. The amino acid substitution of p33^ING1b are shown to the right.

Figure 3 LOH analysis on chromosome 13q33-34 in PCs. A: Submatic representation of LOH distribution;●LOH○ without LOH; a: cancer; b: matched cancer; B: Analysis of D13S1315; C: LOH analysis of D13S261; D: LOH analysis of DS42490; E: LOH analysis of D13S1047.